Propagation in vitro of Quishuar (Buddleja incana RUÍZ & PAV).

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Paola Jiménez Enriquez https://orcid.org/0000-0001-7026-8537
Paulo Barrera Aguilar https://orcid.org/0000-0003-1465-1114
Laura Elizabeth Huachi Espín http://orcid.org/0000-0002-8413-3534
Antonio Vera Zambrano https://orcid.org/0000-0002-9136-8825
Carlos Caicedo Vargas https://orcid.org/0000-0002-2777-5282

Keywords

contamination, in vitro, micropropagation, oxidation, Quishuar

Abstract

Quishuar is a forest species valued for its uses. Intensive exploitation and unsatisfied offer have been limiting factors for reforestation programs. Micropropagation is a technique to eradicate this problem since its goal is to produce many plants in less time. The aim of this study was develop in vitro disinfection; establishment, and multiplication protocols, for this purpose seeds and buds of nursery plants were cultured. In the first phase with seeds, exposed to kilol and benomil, along with NaOCl 3 % (10 min), resulted germination percentage was 100 % in MS (Murashige y Skoog) and contamination and oxidation percentage to 0 %. On the other hand, shoots with fungicides, antioxidants and NaOCl 1 % (10 min), didn´t showed contamination and oxidation. Sprouting percentage was 100 % in WPM (Woody Plant Medium). In the second phase, the greater bud length (1.95 cm), knots number (1.94 knots) and multiplication rate (2.67) were reported in MS without hormones. Based on the results, protocols were effective for in vitro propagation.
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