Composición química, actividad antioxidante y antimicrobiana del aceite esencial proveniente de la hojas de Piper pubinervulum C. DC Piperaceae.
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Publicado:
Jul 1, 2016
Palabras clave:
Piper pubinervulum, GC/EM, Actividad antioxidante, MIC.
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Resumen
Piper puvinervulum C. DC., es una planta medicinal utilizada por los indígenas amazónicos del sur del Ecuador, las hojas poseen características aromáticas. El objetivo de la presente investigación es la de extraer y analizar las propiedades químicas y de actividad biológica del aceite esencial proveniente de las hojas.El estudio de composición química a través de GC-EM y RMN 1H arrojó la detección de 44 constituyentes dentro de los cuales b cariofileno, isoeugenol-metil éter, asarona y el nerolidol fueron los mayoritarios. La Actividad antioxidante del aceite fue evaluada por los métodos DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) y la fotoquimioluminiscencia (PCL). Adicionalmente se realizaron estudios de actividad empleando (HP)TLC-DPPH bioautográficos. La actividad antimicrobiana fue evaluada por el método de difusión de disco en dos bacterias Gram+, dos bacterias Gram- y dos levaduras. Los resultados más interesantes se producen con las dos levaduras Candida tropicalis (MIC 0,77 mg/ml) y Candida albicans (MIC 0,33mg/ml) donde la actividad fue similar al aceite esencial de Thymus vulgaris el estándar natural de referencia. Los buenos resultados con respecto a la actividad antifúngica nos llevan a concluir que el aceite esencial podría ser usado con esta finalidad.
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Referencias
Adam, K.; Sivropoulou, A.; Kokkini, S.; Lanaras, T.; Arsenakis, M. (1998). Antifungal activities of Origanum vulgare subsp. hirtum, Mentha spicata, Lavandula angustifolia, and Salvia fruticosa essential oils against human pathogenic fungi. Journal of Agricultural and Food Chemistry, 46: 1739-1745.
Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry. 4ta ed. Allured publishing corporation.
Adams, R. P. (2012). Identification of essential oils by ion trap mass spectroscopy. Academic Press.
Barbosa, Q. P.; da Câmara, C. A.; Ramos, C. S.; Nascimento, D. C.; Lima-Filho, J. V.; Guimarães, E. F. (2012). Chemical composition, circadian rhythm and antibacterial activity of essential oils of Piper divaricatum: a new source of safrole. Química Nova, 35: 1806-1808.
Benkeblia, N. 2004. Antimicrobial activity of essential oil extracts of various onions (< i> Allium cepa) and garlic (< i> Allium sativum). LWT-Food Science and Technology, 37: 263-268.
BioPhat.Explore (2013) (http://www.molecular-networks.com/biopath3/biopath/mols/) Acceso 19/12/2013.
Chen, Y.; Wang, M..; Rosen, R. T.; Ho, C. T. (1999). 2, 2-Diphenyl-1-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum Thunb. Journal of Agricultural and Food Chemistry, 47:2226-2228.
Diego, L. (2012). Chemical composition and antifungal activity of Piper auritum Kunth and Piper holtonii C. DC. against phytopathogenic fungi. Chilean Journal of Agricultural Research, 72: 4.
Durst, H.D.; Gokel, G.W. (1985). Química orgánica experimental. 1a Ed. Ed Reverte, Barcelona, 600 pp.
Guerrini, A.; Sacchetti, G.; Rossi, D.; Paganetto, G.; Muzzoli, M., Andreotti, E.; Bruni, R. (2009). Bioactivities of< i> Piper aduncum L. and< i> Piper obliquum Ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador. Environmental toxicology and pharmacology, 27: 39-48.
Jaramillo, M. A.; Manos, P. S. (2001). Phylogeny and patterns of floral diversity in the genus Piper (Piperaceae). American Journal of Botany, 88: 706-716.
Jørgensen, P. M.; León-Yánez, S. (1999). Catálogo de las plantas vasculares del Ecuador. Monographs in Systematic Botany from the Missouri Botanical Garden, 75, 1-1181.
Lee, S. J.; Han, J. I.; Lee, G. S.; Park, M. J.; Choi, I. G.; Na, K. J.; Jeung, E. B. (2007). Antifungal effect of eugenol and nerolidol against Microsporum gypseum in a guinea pig model. Biological and Pharmaceutical Bulletin, 30: 184-188.
Macbride, J. F. (1960). Flora of Peru (Vol. 8). Chicago, IL: Field museum of natural history.
Miller, N. J.; Sampson, J.; Candeias, L. P.; Bramley, P. M.; Rice-Evans, C. A. (1996). Antioxidant activities of carotenes and xanthophylls. FEBS letters, 384: 240-242.
NIST/02. (2012). Mass Spectral Library, United States Government.
Noriega Rivera, P. F.; Guerrini, A.; Ankuash Tsamaraint, E. (2014). Composición química del aceite esencial de hojas de Siparuna schimpffii Diels (limoncillo). Revista Cubana de Plantas Medicinales, 19: 128-137.
Oliveira, G. L.; Moreira, D. D. L.; Mendes, A. D. R.; Guimaraes, E. F.; Figueiredo, L. S.; Kaplan, M. A. C.; Martins, E. R. (2013). Growth study and essential oil analysis of Piper aduncum from two sites of Cerrado biome of Minas Gerais State, Brazil. Revista Brasileira de Farmacognosia, 23: 743-753.
Plant Caterpillar Parasitoid Interactions, 2013. (http://caterpillars.lifedesks.org/pages/298118). Acceso en 26/10/2013.
Popov, I.; Lewin, G. (1996). “Photochemiluminescent detection of antiradical activity; IV: testing of lipid-soluble antioxidants”, J Biochem Biophys Methods, 31: 1-8.
Prabuseenivasan, S.; Jayakumar, M.; Ignacimuthu, S. (2006). In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine, 6: 39.
BioPhat.Explore 2013
(https://translate.google.com.ec/#en/es/field%20code%20changed). Acceso 19/12/2013.
Purkayastha, S.; Narain, R.; Dahiya, P. (2012). Evaluation of antimicrobial and phytochemical screening of Fennel, Juniper and Kalonji essential oils against multi drug resistant clinical isolates. Asian pacific journal of tropical Biomedicine, 2: 1625-1629.
Rossi, D.; Guerrini, A.; Maietti, S.; Bruni, R.; Paganetto, G.; Poli, F.; Sacchetti, G. (2011). Chemical fingerprinting and bioactivity of Amazonian Ecuador< i> Croton lechleri Müll. Arg.(Euphorbiaceae) stem bark essential oil: A new functional food ingredient?. Food chemistry, 126: 837-848.
Sabulal, B.; Dan, M.; Kurup, R.; Pradeep, N. S.; Valsamma, R. K.; George, V. (2006). Caryophyllene-rich rhizome oil of Zingiber nimmonii from South India: Chemical characterization and antimicrobial activity. Phytochemistry, 67: 2469-2473.
Sadgrove, N. J.; Telford, I. R.; Greatrex, B. W.; Jones, G. L. (2014). Composition and antimicrobial activity of the essential oils from the< i> Phebalium squamulosum species complex (Rutaceae) in New South Wales, Australia. Phytochemistry, 97: 38-45.
Sauter, I. P.; Rossa, G. E.; Lucas, A. M.; Cibulski, S. P.; Roehe, P. M.; da Silva, L. A. A.; von Poser, G. L. (2012). Chemical composition and amoebicidal activity of< i> Piper hispidinervum(Piperaceae) essential oil. Industrial Crops and Products, 40: 292-295.
Scartezzini, P.; Antognoni, F.; Raggi, M. A.; Poli, F.; Sabbioni, C. (2006). Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of< i> Emblica officinalis Gaertn. Journal of ethnopharmacology, 104:113-118.
Ziosi P.; Manfredini S.; Vertuani S. (2010). Evaluating essential oils in cosmetics: antioxidant capacity and functionality; Cosmetics and toiletries, 125: 32-40.
Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry. 4ta ed. Allured publishing corporation.
Adams, R. P. (2012). Identification of essential oils by ion trap mass spectroscopy. Academic Press.
Barbosa, Q. P.; da Câmara, C. A.; Ramos, C. S.; Nascimento, D. C.; Lima-Filho, J. V.; Guimarães, E. F. (2012). Chemical composition, circadian rhythm and antibacterial activity of essential oils of Piper divaricatum: a new source of safrole. Química Nova, 35: 1806-1808.
Benkeblia, N. 2004. Antimicrobial activity of essential oil extracts of various onions (< i> Allium cepa) and garlic (< i> Allium sativum). LWT-Food Science and Technology, 37: 263-268.
BioPhat.Explore (2013) (http://www.molecular-networks.com/biopath3/biopath/mols/) Acceso 19/12/2013.
Chen, Y.; Wang, M..; Rosen, R. T.; Ho, C. T. (1999). 2, 2-Diphenyl-1-picrylhydrazyl radical-scavenging active components from Polygonum multiflorum Thunb. Journal of Agricultural and Food Chemistry, 47:2226-2228.
Diego, L. (2012). Chemical composition and antifungal activity of Piper auritum Kunth and Piper holtonii C. DC. against phytopathogenic fungi. Chilean Journal of Agricultural Research, 72: 4.
Durst, H.D.; Gokel, G.W. (1985). Química orgánica experimental. 1a Ed. Ed Reverte, Barcelona, 600 pp.
Guerrini, A.; Sacchetti, G.; Rossi, D.; Paganetto, G.; Muzzoli, M., Andreotti, E.; Bruni, R. (2009). Bioactivities of< i> Piper aduncum L. and< i> Piper obliquum Ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador. Environmental toxicology and pharmacology, 27: 39-48.
Jaramillo, M. A.; Manos, P. S. (2001). Phylogeny and patterns of floral diversity in the genus Piper (Piperaceae). American Journal of Botany, 88: 706-716.
Jørgensen, P. M.; León-Yánez, S. (1999). Catálogo de las plantas vasculares del Ecuador. Monographs in Systematic Botany from the Missouri Botanical Garden, 75, 1-1181.
Lee, S. J.; Han, J. I.; Lee, G. S.; Park, M. J.; Choi, I. G.; Na, K. J.; Jeung, E. B. (2007). Antifungal effect of eugenol and nerolidol against Microsporum gypseum in a guinea pig model. Biological and Pharmaceutical Bulletin, 30: 184-188.
Macbride, J. F. (1960). Flora of Peru (Vol. 8). Chicago, IL: Field museum of natural history.
Miller, N. J.; Sampson, J.; Candeias, L. P.; Bramley, P. M.; Rice-Evans, C. A. (1996). Antioxidant activities of carotenes and xanthophylls. FEBS letters, 384: 240-242.
NIST/02. (2012). Mass Spectral Library, United States Government.
Noriega Rivera, P. F.; Guerrini, A.; Ankuash Tsamaraint, E. (2014). Composición química del aceite esencial de hojas de Siparuna schimpffii Diels (limoncillo). Revista Cubana de Plantas Medicinales, 19: 128-137.
Oliveira, G. L.; Moreira, D. D. L.; Mendes, A. D. R.; Guimaraes, E. F.; Figueiredo, L. S.; Kaplan, M. A. C.; Martins, E. R. (2013). Growth study and essential oil analysis of Piper aduncum from two sites of Cerrado biome of Minas Gerais State, Brazil. Revista Brasileira de Farmacognosia, 23: 743-753.
Plant Caterpillar Parasitoid Interactions, 2013. (http://caterpillars.lifedesks.org/pages/298118). Acceso en 26/10/2013.
Popov, I.; Lewin, G. (1996). “Photochemiluminescent detection of antiradical activity; IV: testing of lipid-soluble antioxidants”, J Biochem Biophys Methods, 31: 1-8.
Prabuseenivasan, S.; Jayakumar, M.; Ignacimuthu, S. (2006). In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine, 6: 39.
BioPhat.Explore 2013
(https://translate.google.com.ec/#en/es/field%20code%20changed). Acceso 19/12/2013.
Purkayastha, S.; Narain, R.; Dahiya, P. (2012). Evaluation of antimicrobial and phytochemical screening of Fennel, Juniper and Kalonji essential oils against multi drug resistant clinical isolates. Asian pacific journal of tropical Biomedicine, 2: 1625-1629.
Rossi, D.; Guerrini, A.; Maietti, S.; Bruni, R.; Paganetto, G.; Poli, F.; Sacchetti, G. (2011). Chemical fingerprinting and bioactivity of Amazonian Ecuador< i> Croton lechleri Müll. Arg.(Euphorbiaceae) stem bark essential oil: A new functional food ingredient?. Food chemistry, 126: 837-848.
Sabulal, B.; Dan, M.; Kurup, R.; Pradeep, N. S.; Valsamma, R. K.; George, V. (2006). Caryophyllene-rich rhizome oil of Zingiber nimmonii from South India: Chemical characterization and antimicrobial activity. Phytochemistry, 67: 2469-2473.
Sadgrove, N. J.; Telford, I. R.; Greatrex, B. W.; Jones, G. L. (2014). Composition and antimicrobial activity of the essential oils from the< i> Phebalium squamulosum species complex (Rutaceae) in New South Wales, Australia. Phytochemistry, 97: 38-45.
Sauter, I. P.; Rossa, G. E.; Lucas, A. M.; Cibulski, S. P.; Roehe, P. M.; da Silva, L. A. A.; von Poser, G. L. (2012). Chemical composition and amoebicidal activity of< i> Piper hispidinervum(Piperaceae) essential oil. Industrial Crops and Products, 40: 292-295.
Scartezzini, P.; Antognoni, F.; Raggi, M. A.; Poli, F.; Sabbioni, C. (2006). Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of< i> Emblica officinalis Gaertn. Journal of ethnopharmacology, 104:113-118.
Ziosi P.; Manfredini S.; Vertuani S. (2010). Evaluating essential oils in cosmetics: antioxidant capacity and functionality; Cosmetics and toiletries, 125: 32-40.